Which molecule is produced as a byproduct during the conversion of citrate to isocitrate?

During the conversion of citrate to isocitrate in the citric acid cycle, no byproducts are released. This step involves the enzyme aconitase, which catalyzes the isomerization of citrate into isocitrate. The reaction occurs through an intermediate known as cis-aconitate but does not produce any additional molecules as byproducts.

In biochemical pathways, byproducts typically result from a reaction that includes the release of gas, energy carriers, or other small molecules, but this particular transformation solely rearranges the citrate molecule without generating any extras. Hence, the correct assertion is that no byproduct is released in this step.