Which intermediate is produced after citrate during the Citric Acid Cycle?

After citrate is formed in the Citric Acid Cycle, the next intermediate produced is cis-aconitate. This transformation is catalyzed by the enzyme aconitase, which facilitates the conversion of citrate into cis-aconitate through the removal and subsequent addition of water.

Citrate undergoes isomerization to form cis-aconitate before it is further converted into isocitrate in the subsequent step of the cycle. Understanding this sequence is crucial because it highlights the importance of enzyme-catalyzed transformations in the cycle and the importance of spatial and chemical rearrangements in metabolic pathways.

The other options represent intermediates that occur later in the cycle and are not directly produced from citrate. The sequence of the Citric Acid Cycle is linear and each step culminates in the formation of distinct intermediates. Therefore, the knowledge of the pathway and the order of transformations is essential for grasping how the cycle progresses.