What is the function of aconitase in the Citric Acid Cycle?

Aconitase plays a crucial role in the Citric Acid Cycle by facilitating the conversion of citrate to isocitrate. This reaction is essential for the proper functioning of the cycle, as isocitrate is the next substrate that continues through subsequent steps. The conversion involves an intermediate known as cis-aconitate, where aconitase catalyzes the dehydration of citrate to form cis-aconitate and then rehydrates the intermediate to produce isocitrate. This isomerization step allows the cycle to progress efficiently and ensures that the biochemical pathways leading to energy production can continue effectively.

The other functions mentioned in the options relate to different enzymes involved in the cycle, but they are not associated with the specific action of aconitase. Understanding the specific substrate and product of the aconitase reaction is key to comprehending the overall mechanics of the Citric Acid Cycle.