During which conversion is FADH2 produced in the Citric Acid Cycle?

In the Citric Acid Cycle, FADH2 is produced during the conversion of succinate to fumarate. This specific step is catalyzed by the enzyme succinate dehydrogenase, which facilitates the oxidation of succinate. As succinate is oxidized, it loses hydrogen atoms, and FAD (flavin adenine dinucleotide) is reduced to FADH2. This process also contributes to the overall energy yield of the cycle, as FADH2 can later enter the electron transport chain to generate ATP.

The production of FADH2 at this step is significant because it reflects the biochemical pathway of extracting energy from the oxidation of substrates in the cycle, facilitating the overall metabolic process.